Moskovskiy A., Lyubovtseva L., Viktorov V., Moskovskaya O., Tsyganov V., Petukhov D.
The study of immunocompetent cells populations in inflamed dental pulp
The aim of the present study was to investigate the quantitative composition and functional status of dental pulp immune cells in patients with pulpitis and periodontitis. Operating biopsies of dental pulp from patients with caries, acute and chronic pulpitis in combination with periodontal disease of varying severity were used in the study. Immunohistochemical examination was carried out by three-stage indirect immunoassay using primary mabs against antigenic markers of immune cells – CD3+ T-lymphocytes, CD20+ B-lymphocytes, CD68+ macrophages. Visual assessment of antigenic markers expression was studied in the pulp tissue using the light microscope Nikon Eclipse E400 with Digital CCD Color Camera KCC-310ND / PD. The intact tooth pulp is highly immunocompetent due to the presence of CD3+, CD20+ and CD68+ cells, which supports both cellular and humoral immunity. The number of CD68+ cells peaks in acute process in the dental pulp and periodontium, which is caused by infiltration of phagocytes.CD3+ and CD20+ cells revealed in the pulp tissues at later stages of inflammation, which indicates the involvement of cellular and humoral immune response. The number of immune cells is reduced in dental pulp and periodontium at chronic inflammation, but does not reach the control group values.
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- Moskovsky Alexander Vladimirovich
- doctor of medical sciences, orthopaedic dentistry and orthodontics department professor, Chuvash State University, Russia, Cheboksary (firstname.lastname@example.org; )
- Viktorov Vladimir Nikolaevich
- candidate of medical sciences, orthopaedic dentistry and orthodontics department associate professor , Chuvash State University, Russia, Cheboksary )
- Moskovskaya Olesya I.
- Candidate of Biological Sciences, Associate Professor of the Department of Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary )
- Petukhov Dmitry Ivanovich
- orthopaedic dentistry department assistant, Chuvash State University, Russia, Cheboksary )
- Tsyganov Valery Petrovich
- orthopaedic dentistry department assistant , Chuvash State University, Russia, Cheboksary )
- Lubovtseva Lubov Alekseevna
- doctor of biological sciences, professor, general and clinical morphology and forensic medicine department head, Chuvash State University, Russia, Cheboksary )
Moskovskiy A., Lyubovtseva L., Viktorov V., Moskovskaya O., Tsyganov V., Petukhov D. The study of immunocompetent cells populations in inflamed dental pulp [Electronic resource] // Acta medica Eurasica. – 2016. – №2. P. 38-41. – URL: http://acta-medica-eurasica.ru/en/single/2016/2/7/.