Single article

Fufaeva A., Kozlov A., Sapozhnikov S., Petrova Yu., Alexandrova V.

Influence of red grape wine and its combinations with hexoses in standard amyloid disease model formation

Keywords: red dry wine, the red wine polyphenols, amyloidosis, amyloid kidney damage, amyloid liver damage

The research aim was to study the effect of red wine polyphenols and their combination with simple six-carbon keto-alcohols on amyloid disease development in young mice, reproduced using a standardized model of amyloidosis. In the experiment on one-month-old age young male mice, formation of amyloid disease model by subcutaneous injection of 0,3 ml of a 10% aqueous solution of soy cream substitute TU 9199-004-58706213-10, (15 injections every other day without aseptic treatment) caused generalized amyloidosisof the liver, kidneys and the spleen with a significant increase in wet weight of organs to 22,0±0,1%, 35,0±0,5 and 66,0±0,5%, respectively. In addition, a marked formation of amyloid deposits in the tissues of organs was observed, which was established by staining 5-micron paraffin sections with Congo red. Intake of red dry wine Cabernet (Crimea) and its combination with 5% fructose against the background of amyloid model formation statistically significantly reduced both the change in the wet weight of organs and histologically controlled deposition of amyloid in the tissue. We concluded that red dry grape wine consumption in the regime of free daily intake prevents the development of histomorphological changes in the organs under investigation (liver, kidney, spleen) and reduces the amount of Congo-positive substance in these organs, the effect is enhanced by combining red wine with fructose.


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About authors

Alexandrova Vera Yurievna
3th year student, Chuvash State University, Russia, Cheboksary (; )
Fufaeva Alena I.
Physician, Department of Otorhinolaryngology and Maxillofacial Surgery, Republican Pediatric Clinical Hospital, Russia, Cheboksary (; ORCID:
Kozlov Vadim A.
Doctor of Biological Sciences, Candidate of Medical Sciences, Professor of the Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (; ORCID:
Petrova Julia Victorovna
3th year student, Chuvash State University, Russia, Cheboksary (; )
Sapozhnikov Sergey P.
Doctor of Medical Sciences, Head of the Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (; ORCID:

Article link

Fufaeva A., Kozlov A., Sapozhnikov S., Petrova Yu., Alexandrova V. Influence of red grape wine and its combinations with hexoses in standard amyloid disease model formation [Electronic resource] // Acta medica Eurasica. – 2018. – №1. P. 42-51. – URL: