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Moroz S.E., Lavrentieva E.A., Kastyro I.V., Khlystalov M.V., Ibadullayeva S.S., Dyachenko Yu.E., Kartasheva A.F.
Histological Changes in Autografts of Adipose Tissue in Rats in the Middle Postoperative Period
Keywords: autologous fat, inflammation, lipofilling, modeling of lipofilling
Autotransplantation of adipose tissue is a widely used technique in aesthetic and reconstructive surgery for correcting atrophic changes and scar deformities. Adipose tissue is a biologically active substance and its function goes far beyond fat deposition. Objective. To identify histological changes in autografts of adipose tissue and surrounding tissues in rats after various methods of fat treatment 30 days after surgical interventions. Materials and methods. The animals were divided into five groups, with six individuals in each group. Group 1 consisted of intact animals (no exposure); Group 2 served as the control (saline solution); Group 3 included animals with a subdermal implantation of fat, previously extracted from a skin incision in the groin area, through a 1 mm diameter incision. In Group 4, fat crushed with a scalpel was injected under the skin with a syringe once. In Group 5, animals were implanted with fat crushed using a shredder nozzle in a Luer Lock syringe. Four implantations were performed with a volume of 0.05 ml. The sections were stained using the Mallory method, hematoxylin and eosin, methylene blue. The Mann-Whitney U test was used to assess statistically significant differences. Results. Morphometric parameters of the dermis showed an increase in its thickness, mainly of the mesh layer, as well as, physiologically significant increase in the proportion of microcirculatory vessels in the skin of animals in the experimental groups. The number of sebaceous gland profiles was slightly higher in the experimental groups compared to the control groups (p < 0.05). The thickness of the fat autograft was significantly greater in Group 3 (p < 0.001), where a large fat fragment was implanted, however, in this group, almost all rats had pathological reactions in the form of leukocyte (primary lymphocytic) infiltrations and varying degrees of necrosis. Infiltrations containing lymphocytes and fibroblast-like cells were also observed in animals of groups 4 and 5, but were significantly less pronounced than in Group 3. In Group 5, relatively large fat fragments surrounded by lymphocytic infiltrates were detected in the skin of only two rats, in other animals, fat fragments of microscopic size were found, which, as a rule, were integrated with the adipose tissue of the transplant site. Conclusions. The introduction of autologous fat can not only lead to its gradual degradation, but also can stimulate the formation of adipose tissue in the area of administration. The components of the fat graft (lipids) become part of the newly formed adipose tissue. This process is more effective when the size of the injected graft particles is smaller.
References
- Cheng C., Wang W., Pu L.L.Q. Targeted fat grafting: the concept of anatomic restoration for safe and optimal facial rejuvenation. Aesthetic Surg J, 2020, 40(12), pp. 705–707.
- Kastyro I.V., Kostyaeva M.G., Torshin V.I. et al. The Study of The Effect Of Stress on The Morphological Changes in The Hippocampus after Surgical Interventions in the Nasal Region. Morfologia, 2019, vol. 156(4), pp. 25–34.
- Khazaei S., Keshavarz G., Bozorgi A. et al. Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods. Cell Tissue Bank, 2022, 23(1), pp. 1–16.
- Khouri R.K., Khouri R.K. Current Clinical Applications of Fat Grafting. Plast Reconstr Surg, 2017, 140(3), pp. 466–486.
- Negenborn V.L., Groen J.W., Smit J.M. et al. The use of autologous fat grafting for treatment of scar tissue and scar-related conditions: A systematic review. Plastic and Reconstructive Surgery, 2016, 137(1), pp. 31–43.
- Nishimura T., Hashimoto H., Nakanishi I. et al. Microvascular angiogenesis and apoptosis in the survival of free fat grafts. Laryngoscope, 2000, 110(8), pp. 1333–1338.
- Peng Z., Tang P., Zhao L. et al. Advances in biomaterials for adipose tissue reconstruction in plastic surgery. Nanotechnology Reviews, 2020, 9(1), pp. 385–395.
- Rigotti G., Marchi A., Galie M. et al. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: A healing process mediated by adipose-derived adult stem cells. Plastic and Reconstructive Surgery, 2007, 119(5), pp. 1409–1424.
- Sinno S., Mehta K., Reavey P.L. et al. Current trends in facial rejuvenation: An assessment of ASPS members’ use of fat grafting during face lifting. Plastic and Reconstructive Surgery, 2015, 136(1), pp. 20–30.
- Topcu A., Aydin O. E., Mehtat U. et al. Increasing the viability of fat grafts by vascular endothelial growth factor. Arch Facial Plast Surg, 2012, 14(4), pp. 270–276.
- Shamloul G., Khachemoune A. An updated review of the sebaceous gland and its role in health and diseases Part 2: Pathophysiological clinical disorders of sebaceous glands. Dermatol Ther, 2021, vol. 34(2),
About authors
- Moroz Svetlana E.
- Post-Graduate Student, Department of Plastic Surgery, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (sve5moroz@gmail.com; ORCID: https://orcid.org/0000-0002-3892-0596)
- Lavrentieva Elina A.
- Post-Graduate Student, Department of Plastic Surgery, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (laveelina@ya.ru; ORCID: https://orcid.org/0000-0002-1641-5863)
- Kastyro Igor V.
- Doctor of Medical Sciences, Professor, Department of Plastic Surgery, Department of Clinical Physiology and Non-medicinal Methods of Treatment, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (ikastyro@gmail.com; ORCID: https://orcid.org/0000-0001-6134-3080)
- Khlystalov Maxim V.
- Post-Graduate Student, Department of Plastic Surgery, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (hlystalov@inbox.ru; ORCID: https://orcid.org/0009-0002-6766-8323)
- Ibadullayeva Svetlana S.
- Post-Graduate Student, Department of Plastic Surgery, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (ibadullayeva00@gmail.com; ORCID: https://orcid.org/0009-0006-9651-949X)
- Dyachenko Yulia E.
- Resident of the Department of Neurosurgery, N.N. Burdenko National Medical Research Center of Neurosurgery, Russia, Moscow (julika-98@yandex.ru; ORCID: https://orcid.org/0000-0001-8469-6073)
- Kartasheva Alla F.
- Doctor of Medical Sciences, Professor, Department of Plastic Surgery, Peoples' Friendship University of Russia named after Patrice Lumumba, Russia, Moscow (kartasheva_af@pfur.ru; ORCID: https://orcid.org/0000-0002-8533-301X)
Article link
Moroz S.E., Lavrentieva E.A., Kastyro I.V., Khlystalov M.V., Ibadullayeva S.S., Dyachenko Yu.E., Kartasheva A.F. Histological Changes in Autografts of Adipose Tissue in Rats in the Middle Postoperative Period [Electronic resource] // Acta medica Eurasica. – 2024. – №3. P. 26-33. – URL: https://acta-medica-eurasica.ru/en/single/2024/3/4/. DOI: 10.47026/2413-4864-2024-3-26-33.