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DOI: 10.47026/2413-4864-2023-1-160-170
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Timofeeva N.Yu., Bubnova N.V., Stomenskaya I.S., Struchko G.Yu., Kostrova O.Yu.

Methods of Visualization of Mast Cells (Literature Review)

Keywords: mast cells, Unna staining, toluidine blue, alcian blue, safranin, tryptase

Mast cells play an important role in many processes occurring in the human body. These include inflammation, allergic reactions, parasitic infections, carcinogenesis and others. Therefore, the detection of mast cells plays an important role in the diagnosis of pathological conditions. The purpose of the study is to summarize data on imaging methods of mast cells. Materials and methods. Review of available literature sources published in Elibrary and PubMed. Results. There are several major groups of methods for finding mast cells: histochemical, enzyme-linked histochemical and immunohistochemical. All are based on the ability to detect specific substances contained in mast cell granules. Histochemical methods include Unno, alcian blue and safranin staining of mast cells and others. The most modern method is mast cell visualisation using immunohistochemical reactions based on antigen-antibody reactions. Conclusion. This review presents the most common methods of mast cell staining, differing in difficulty of performance, specificity in relation to mast cells and staining cost.

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About authors

Timofeeva Natalya Yu.
Senior Lecturer, Department of Instrumental Diagnostics Department with a Course of Phthisiology, Chuvash State University, Russia, Cheboksary (bla11blabla@yandex.ru; ORCID: https://orcid.org/0000-0002-7596-0132)
Bubnova Natalia V.
Senior Lecturer, Department of Instrumental Diagnostics Department with a Course of Phthisiology, Chuvash State University, Russia, Cheboksary (natalia210485@yandex.ru; ORCID: https://orcid.org/0000-0002-2505-0827)
Stomenskaya Irina S.
Candidate of Medical Sciences, Assistant Professor, Department of the Instrumental Diagnostics with a Course of Phthisiology, Chuvash State University, Russia, Cheboksary (irina.stomenskaja@gmail.com; ORCID: https://orcid.org/0000-0001-7332-4477)
Struchko Gleb Yu.
Doctor of Medical Sciences, Professor, Head of Normal and Topographic Anatomy Department, Chuvash State University, Russia, Cheboksary (glebstr@mail.ru; ORCID: https://orcid.org/0000-0002-0549-5116)
Kostrova Olga Yu.
Candidate of Medical Sciences, Assistant Professor, Head of Department of the Instrumental Diagnostics with a Course of Phthisiology, Chuvash State University, Russia, Cheboksary (evkbiz@yandex.ru; ORCID: https://orcid.org/0000-0002-7057-9834)

Article link

Timofeeva N.Yu., Bubnova N.V., Stomenskaya I.S., Struchko G.Yu., Kostrova O.Yu. Methods of Visualization of Mast Cells (Literature Review) [Electronic resource] // Acta medica Eurasica. – 2023. – №1. P. 160-170. – URL: https://acta-medica-eurasica.ru/en/single/2023/1/18/. DOI: 10.47026/2413-4864-2023-1-160-170.

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