Changes in the Histamine Status of the Internal Organs of Laboratory Animals Depending on the Silicon Concentration in Drinking Water

DOI: 10.47026/2413-4864-2023-1-83-92

UDC: [591.4-932.342:577.175.824]:[546.28:613.31]
BBC: [Е0*725.29-641.9:Г124.2]:Е623.362.423.2*739.1*022.3

Gordova V.S., Grigoreva E.A., Sergeeva V.E., Smirnova N.V., Karyshev P.B.

Changes in the Histamine Status of the Internal Organs of Laboratory Animals Depending on the Silicon Concentration in Drinking Water

Keywords: silicon, amorphous silicon dioxide, liver, spleen, testes, histamine, biogenic amines

The results of a study of the histamine status of the internal organs of Djungarian hamsters when silicon was taken with drinking water are presented. The aim of the study was to assess the histamine status of the liver, spleen and testicles of Djungarian hamsters when silicon was ingested with drinking water for three months at various concentrations. Material and methods. The hamsters were kept in the vivarium on a standard diet with free access to drinking water for three months. Hamsters of the control group (n = 3) received bottled drinking water, hamsters of the experimental groups received the same bottled water with the addition of sodium metasilicate 9-hydrate at a concentration of 10 mg/l in terms of silicon (the first experimental group, n = 3) and 20 mg/l in terms of silicon (the second experimental group, n = 3). To detect and quantify histamine in organs and tissues in cryostat sections of the liver, spleen and testicles, the fluorescent-histochemical Cross method was used. A general blood test and a blood test for glucose and cholesterol were also performed. Results and their discussion. A general blood test of hamsters that received drinking water with different concentrations of silicon did not reflect the effect of microelement intaked into the organism, while, depending on the concentration of silicon in water, the average blood glucose level tended to increase, and the cholesterol level tended to decrease. The obtained results do not contradict our previous studies in that direction. It has been shown that the concentration of silicon in drinking water from 10 mg/l hardly noticeably affects the histamine status of such organs as the liver, spleen and testes: only the intensity of histamine luminescence in macrophages of the red pulp increases statistically significantly. When silicon enters the body with drinking water at a concentration of 20 mg/l, the histamine status of organs changes more noticeably, the cells surrounding the central veins and the interstitial histamine-containing testes cells are “involved” into the reaction, which is visually reflected in the luminescent morphology of the investigated organs. Conclusions. The intake of silicon at a concentration of 10 mg/l and 20 mg/l for three months affects the histamine status of the liver, spleen and testes of Djungarian hamsters, while the indicators of the general blood test do not change.

References

Zaitseva N.V., Zemlyanova M.A., Zvezdin V.N. et al. Vliyanie nanochastits dioksida kremniya na morfologiyu vnutrennikh organov u krys pri peroral’nom vvedenii [Influence of silicon dioxide nanoparticles on the morphology of internal organs in rats after oral administration]. Analiz riska zdorov’yu, 2016, no. 4, pp. 80–94. DOI: 10.21668/health.risk/2016.4.10.
Gordova V.S., Sergeeva V.E., Korshunova A.I. et al. Gistaminsoderzhashchie kletki limfoidnykh organov laboratornykh gryzunov v eksperimente [Histamine containing cells of lymphoid organs of laboratory rodents in experiment]. Vestnik novykh meditsinskikh tekhnologii, vol. 25, no. 3, pp. 107–115.
Gordova V.S., Sergeeva V.E., Karyshev P.B. Gistaminsoderzhashchie struktury limfoidnykh organov laboratornykh krys pri dlitel’nom postuplenii kremniya s pit’evoi vodoi [Histamine-Containing Structures of Lymphoid Organs of Laboratory Rats with Long-Term Silicon Intake with Drinking Water]. In: Materialy Mezhdunarodnoi nauchnoi shkoly «Nauka i innovatsii – 2013» ISS «SI-2013» 7-12 iyulya 2013 g. [Proc. of Int. Sci. School “Science and Innovation – 2013”]. Yoshkar-Ola, 2013, pp. 159–164.
Gordova V.S., Sergeeva V.E., Sapozhnikov S.P. Morfologicheskaya adaptatsiya vnutrennikh organov k postupleniyu v organizm vodorastvorimogo soedineniya kremniya [Morphological adaptation of internal organs to intake of a water-soluble compound silicon]. Cheboksary, 2021, 208 p.
Grigor’eva E.A. Morfologicheskie osobennosti pecheni pri vozdeistvii vodorastvorimogo soedineniya kremniya [Morphological features of the liver when exposed to a water-soluble silicon compound]. Meditsinskii akademicheskii zhurnal, 2016, vol. 16, no. 4, pp. 71–72.
Rakhmanin Yu.A., Egorova N.A., Mihaylova R.I. et al. O gigienicheskom normirovanii soedinenii kremniya v pit’evoi vode (obzor literatury) [On the hygienic rating of silicon compounds in drinking water (literature review)]. Gigiena i sanitariya, 2021, vol. 100, no. 10, pp. 1077–1083.
Salmina A.E. Kharakteristika neitrofilov pri postuplenii v organizm vodorastvorimogo kremniya [Characteristics of neutrophils upon intake of water-soluble silicon]. In: Sbornik materialov Vseros. nauch.- konf. molodykh uchenykh i studentov s mezhdunar. uchastiem «Meditsinskaya Vesna-2015». (g. Moskva, 19 maya 2015 g) [Proc. of Russ. Sci. and Pract. Conf. “Medical Spring-2015”]. Moscow, 2015, p. 455.
Salmina A.E., Gordova V.S. Kharakteristika perifericheskoi krovi krys pri vnutri-vennom vvedenii vodorastvorimogo soedineniya kremniya [Characteristics of the peripheral blood of rats with intravenous administration of a water-soluble silicon compound]. In: Sbornik tezisov XI Mezhdunar. (XX Vseros.) Pirogovskoi meditsinskoi konf. studentov i molodykh uchenykh (Moskva, 19 marta 2016) [Proc. of 11^th (10^th Russ.) Pirogov Medical Conf. of Students and Young Scientists. Moscow, 2016, pp. 239–240.
Sapozhnikov S.P., Gordova V.S., Sergeeva V.E., Kozlov V.A. Coedineniya kremniya i aterogenez (obzor) [Silicon chemicals and atherogenesis (a review)]. Mikroelementy v medicine, 2022, vol. 23, no. 1, pp. 4–13.
Sergeeva V.E., Gordova V.S., Gordon D.S. Lyuminestsentnaya gistokhimiya biogennykh aminov v morfo-funktsional’nom sostoyanii organov i tkanei v eksperimente (nauchno-istoricheskii obzor) [Luminescent hystochemistry of biogenic amines in morphofunctional status of organs and tissues in experiment (scientific and historical overview)]. Acta medica Eurasica, 2017, no. 3, pp. 30–49.
Surov A.V., Feoktistova N.Yu. Biologiya mokhnonogikh khomyachkov i ikh ispol’zovanie v laboratornoi praktike [Biology of legged hamsters and their use in laboratory practice]. Biomeditsina, 2006, no. 2, pp. 52–70.
Smirnova S.S., Grigor’eva E.A., Golenkova V.A. et al. Eozinofily kak vozmozhnye regulyatory urovnya gistamina v pecheni krys [Eosinophiles as possible regulators of histamine level in rat’s liver]. In: Morfologiya v teorii i praktike: materialy Vseros. nauch.-prakt. konf. s mezhdunar. uchastiem, posvyashch.i 95-letiyu so dnya rozhdeniya d-ra med. nauk, professora Diny Semenovny Gordon (Cheboksary, 22 noyabrya 2017 g.) [Proc. of Russ. Sci. Conf. «Morphology in theory and practice»]. Cheboksary, 2017, pp. 221–223.
Abiuso A.M., Berensztein E., Pagotto R.M. et al. H4 histamine receptors inhibit steroidogenesis and proliferation in Leydig cells. The Journal of endocrinology, 2014, vol. 223, no. 3, pp. 241–253.
Jumat M.I., Hayati F., Syed Abdul Rahim S.S., Saupin S. et al. Occupational lung disease: A narrative review of lung conditions from the workplace. Ann Med Surg (Lond), 2021, vol. 64, e102245.
Leung C.C., Yu I.T., Chen W. Lancet, 2012, vol. 379, no. 9830, pp. 2008–2018.
Li X., Li Y., Lv S., Xu H. et al. Long-term respiratory exposure to amorphous silica nanoparticles promoted systemic inflammation and progression of fibrosis in a susceptible mouse model. Chemosphere, 2022, vol. 300, e134633.
Liu J.Y., Sayes C.M. A toxicological profile of silica nanoparticles. Toxicol Res (Camb), 2022, vol. 11, no. 4, pp. 565–582.
Mondillo C., Falus A., Pignataro O., Pap E. Prolonged histamine deficiency in histidine decarboxylase gene knockout mice affects Leydig cell function. Journal of andrology, 2007, vol. 28, no. 1, pp. 86–91.
Murugadoss S., Lison D., Godderis L., Van Den Brule S. et al. Toxicology of silica nanoparticles: an update. Arch Toxicol, 2017, vol. 91, no. 9, pp. 2967–3010.
Safina F., Tanaka S., Inagaki M., Tsuboi K. et al. Expression of L-histidine decarboxylase in mouse male germ cells. J Biol Chem, 2002, vol. 277, no. 16, pp. 14211–14215.

About authors

Gordova Valentina S.: Candidate of Medical Sciences, Associate Professor, Department
of Fundamental Medicine, Immanuel Kant Baltic Federal University, Russia, Kaliningrad (crataegi@rambler.ru; ORCID: https://orcid.org/0000-0001-5109-9862)

Grigoreva Evgeniia A.: Post-Graduate Student, Department of Medical Biology with a course of Microbiology and Virology, Chuvash State University, Russia, Cheboksary (shgrev@yandex.ru; ORCID: https://orcid.org/0000-0003-3626-2750)

Sergeeva Valentina E.: Doctor of Biological Sciences, Professor, Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (kaf-biology@yandex.ru; ORCID: https://orcid.org/0000-0003-3471-5226)

Smirnova Nadezhda V.: Candidate of Biological Sciences, Associate Professor, Head of Department of Medical Biology with course of Microbiology and Virology, Chuvash State University, Russia, Cheboksary (nadyas05@mail.ru; ORCID: https://orcid.org/0000-0001-7346-6301)

Karyshev Pavel B.: Laboratory Assistant, Department of Medical Biology with the Course of Microbiology and Virology, Chuvash State University, Russia, Cheboksary (pkarmol@mail.ru; ORCID: https://orcid.org/0000-0002-7703-8889)

Article link

Gordova V.S., Grigoreva E.A., Sergeeva V.E., Smirnova N.V., Karyshev P.B. Changes in the Histamine Status of the Internal Organs of Laboratory Animals Depending on the Silicon Concentration in Drinking Water [Electronic resource] // Acta medica Eurasica. – 2023. – №1. P. 83-92. – URL: https://acta-medica-eurasica.ru/en/single/2023/1/10/. DOI: 10.47026/2413-4864-2023-1-83-92.

Single article

Gordova V.S., Grigoreva E.A., Sergeeva V.E., Smirnova N.V., Karyshev P.B.