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Kozlov V., Sapozhnikov S., Fufaeva A., Nikolaeva O., Deоmidov E.
Parenteral administration of albumin induces amyloid brain damage
Keywords: human albumin, mice, amyloidosis model, amyloid, congo red, brain, dorsoventral paraffin sections
The aim of the study was to obtain amyloidosis in laboratory mice in experiment with a parenteral administration of human pharmacological albumin. Experimental models of generalized amyloidosis, induced by administration of bovine serum albumin or ovi albumin are known. It is also known that human genuine serum albumin participates in amyloid formation. Nevertheless, in medicine, intravenous administration of human albumin preparations for dehydration in various critical conditions is widely used. Such treatment is considered to be safe, but is it? Young one-month-old mice were injected subcutaneously with soya cream substitute TU 9199-004-58706213-10 (control of amyloidosis formation according to the standard model – KM), or 10% aqueous solution of human pharmacological albumin at a dose of 0.9 ml per day, 14 injections intraperitoneally (experimental group). This caused generalized amyloid involvement of internal organs, including subcortical areas of the brain and cerebellum, which was histologically verified by staining with red Congo. Amyloid deposition square area (SA) in 4 μ-paraffin sections in control group (CG) made: 61,4±15,48%, against 40,18±14,82% in experimental group. Within 30 days after the end of amyloidogens administration, survival was accompanied by SA increase to 51,51±17,92%. In the cerebellum in CG, SA was 44,01±12,86% versus 24,87±10,18% at the same periods of albumin administration and 39,21±14,75% after 30 days of survival. Thus, intraperitoneal human albumin injection in mice causes a progressive amyloid affection of subcortical areas of the cerebrum and cerebellum. It was concluded that albumin and all protein preparations of human blood containing albumin should be excluded from clinical use for parenteral administration.
References
- Kozlov V.A., Sapozhnikov S.P., Sheptukhina A.I., Golenkov A.V. Sravnitel’nyi analiz razlichnykh modelei amiloidoza [Comparative analysis of amyloidosis various models]. Vestnik Rossiiskoi akademii meditsinskikh nauk, 2015, no. 1, pp. 5–11.
- Kozlov V.A., Sapozhnikov S.P., Karyshev P.B., Sheptukhina A.I., Nikolaeva O.V. Model’ sistemnogo amiloidoza u molodykh myshei [Model of systemic amyloidosis in young mice]. Byulleten’ eksperimental’noi biologii i meditsiny, 2016, vol. 162, no. 10, pp. 523–527.
- Kozlov V.A., Sapozhnikov S.P., Sheptukhina A.I., Karyshev P.B. Sposob modelirovaniya eksperimental’nogo amiloidoza u zhivotnykh [A method for modeling experimental amyloidosis in animals]. Patent RF no. 2572721, 2016.
- Kozlov V.A., Sapozhnikov S.P., Sheptukhina A.I., Golenkov A.V. Parametabolizm kak nespetsificheskii modifikator supramolekulyarnykh vzaimodeistvii v zhivykh sistemakh [Parametabolism as a nonspecific modifier of supramolecular interactions in living systems]. Vestnik Rossiiskoi akademii meditsinskikh nauk, 2015, no. 4(70), pp. 397–402.
- Goldschmidt L., Teng P.K., Riek R., Eisenberg D. Identifying the amylome, proteins capable of forming amyloid-like fibrils. Natl. Acad. Sci. USA., 2010, vol. 107, no. 8, pp. 3487–3492. doi: 10.1073/pnas.0915166107.
- Sipe J.D., Benson M.D., Buxbaum J.N., Ikeda S., Merlini G., Saraiva M.J., Westermark P. Amyloid fibril protein nomenclature: 2010 recommendations from the nomenclature committee of the International Society of Amyloidosis. Amyloid., 2010, vol. 17, no. 3–4, pp. 101–104. doi:3109/1350 6129.2010.526812.
- Selkoe D.J. Alzheimer’s disease: genes, proteins, and therapy. Rev., 2001, vol. 81, no. 2, pp. 741–766.
About authors
- Kozlov Vadim A.
- Doctor of Biological Sciences, Candidate of Medical Sciences, Professor of the Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (pooh12@yandex.ru; ORCID: https://orcid.org/0000-0001-7488-1240)
- Fufaeva Alena I.
- Physician, Department of Otorhinolaryngology and Maxillofacial Surgery, Republican Pediatric Clinical Hospital, Russia, Cheboksary (priffetik@bk.ru; ORCID: https://orcid.org/0000-0003-4771-7562)
- Sapozhnikov Sergey P.
- Doctor of Medical Sciences, Head of the Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (adaptogon@mail.ru; ORCID: https://orcid.org/0000-0003-0967-7192)
- Deomidov Evgeny Sergeevich
- candidate of medical sciences, psychiatry, medical psychology and neurology department associate professor, Chuvash State University, Russia, Cheboksary (neurokaf@yandex.ru; )
- Nikolaeva Oksana Vladislavovna
- psychiatry, medical psychology and neurology department clinical resident, Chuvash State University, Russia, Cheboksary (lovely667@mail.ru; )
Article link
Kozlov V., Sapozhnikov S., Fufaeva A., Nikolaeva O., Deоmidov E. Parenteral administration of albumin induces amyloid brain damage [Electronic resource] // Acta medica Eurasica. – 2017. – №4. P. 45-50. – URL: https://acta-medica-eurasica.ru/en/single/2017/4/7/.