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Ilyin M., Dragunov A., Kozlov V., Kichigin V.
Angiogenesis stimulation by introducing thrombocytes-rich autoplasm into ischaemic myocardial zone
Keywords: myocardial infarction, platelet-rich autoplasma, angiogenesis, alkaline phosphatase, succinate dehydrogenase
One month after myocardial infarction modeling under anesthesia by doping the left coronary artery in experimental group of 10 mongrel rabbits, platelet-rich autoplasma in the volume of 2 ml was injected intramurally into the region of post-infarction cardiosclerosis of 5 rabbits. The remaining five animals served as controls. In 1.5 months after repeated surgical intervention, the animals of both groups were euthanized. In experimental group there was a significant increase in the area occupied by the vessels by 50% (p = 0,032), alkaline phosphatase activity by 14,8% (p = 0,032) and succinate dehydrogenase by 7,0% (p = 0,007) in comparison with control group. A strong correlation of the area occupied by vessels with the activity of alkaline phosphatase and succinate dehydrogenase was established, which makes it possible to consider these enzymes’ activity as a sufficiently reliable morphological equivalent of microcirculation intensity.
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About authors
- Ilyin Mikhail Vladimirovich
- normal and topographic anatomy with operative surgery department senior lecturer, Chuvash State University, Russia, Cheboksary (ilyin-mv@mail.ru; )
- Kichigin Vadim Aleksandrovich
- candidate of medical sciences, unit head, Dialysis center, Russia, Cheboksary (vadim-kichigin@mail.ru; )
- Kozlov Vadim A.
- Doctor of Biological Sciences, Candidate of Medical Sciences, Professor of the Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (pooh12@yandex.ru; ORCID: https://orcid.org/0000-0001-7488-1240)
- Dragunov Andrey Gennadievich
- candidate of medical sciences, cardiac surgery department № 2 head, Republican Cardiology Dispensary, Russia, Cheboksary (adragunov@yandex.ru; )
Article link
Ilyin M., Dragunov A., Kozlov V., Kichigin V. Angiogenesis stimulation by introducing thrombocytes-rich autoplasm into ischaemic myocardial zone [Electronic resource] // Acta medica Eurasica. – 2017. – №3. P. 32-38. – URL: https://acta-medica-eurasica.ru/en/single/2017/3/5/.