Single article

DOI: 10.47026/2413-4864-2021-2-28-33

Bortnikova O.A., Pavlova S.I.

Study of the antitumor activity of isoliquiritigenin on the HeLa, PC3, and MCF-7 Tumor Cell Lines

Keywords: isoliquiritigenin, flavonoid, chalcon, antitumor activity, antipropherative activity, tumor cells lines

In 2020, the most common type of malignancy was breast cancer. According to WHO, in 2020, this type of cancer accounted for one in eight new cases of cancer in the world and one in four – among women: this disease was diagnosed in 2.3 million people. One of the factors influencing a high mortality rate among patients with this diagnosis can be attributed to an increase in the level of tumor cells resistance to the chemotherapeutic preparations used, so today it is extremely important to search for new preparations that have high specificity for malignant neoplasms, low toxicity and availability for patients. In this regard, a lot of researchers' attention is attracted by compounds of natural origin, in particular the group of flavonoids, which includes isoliquiritigenin. According to the results of modern studies, this compound can have antitumor activity in vitro and in vivo, affecting the molecular mechanisms of regulating the growth and survival of tumor cells. The aim of this article is to study the antitumor antiproliferative activity of isoliquiritigenin on the HeLa, PC-3, and MCF-7 tumor cells lines in vitro. The antiproliferative activity was evaluated using the MTT test with the exposure of cells with the test compound for 72 hours. As a result of the study, isoliquiritigenin showed a dose-dependent antiproliferative effect on human tumor lines PC-3, MCF-7 in the concentration range 10–4–2,5×10–5 mol/l.

References

  1. Belitskii G.A., Kirsanov K.I., Lesovaya E.A., Yakubovskaya M.G. Mekhanizmy antikantse-rogennogo deistviya flavonoidov [Mechanisms of anticarcinogenic action of flavonoids]. Uspekhi molekulyarnoi onkologii, 2014, 1(1), pp. 56–68.
  2. Pavlova S.I. Issledovanie ekstrakta kornya solodki dlya povysheniya effektivnosti terapii zlokachestvennykh novoobrazovanii: dis. … kand. med. Nauk [Research of licorice root extract to improve the effectiveness of treatment of malignant neoplasms. Cand. Diss.] Moscow, 2005, p. 106.
  3. Tarakhovskii Yu.S., Kim Yu.A., Abdrasilov B.S., Muzafarov N., eds. Flavonoidy: biokhimiya, biofizika, meditsina [Flavonoids: biochemistry, biophysics, medicine]. Pushchino, 2013, p. 310.
  4. Ferlay J., Ervik M., Lam F., Colombet M., Mery L., Piñeros M. et al. Global Cancer Obser-vatory: Cancer Today. Lyon, International Agency for Research on Cancer, 2020. Available at: https://gco.iarc.fr/today.
  5. Mather J.P., Roberts P.E. Introduction to cell and tissue culture. Theory and technique. New York, Plenum Press, 1998, pp. 175–194.
  6. Peng F., Du Q., Peng C., Wang N., Tang H., Xie X., Shen J., Chen J. A review: the pharma-cology of isoliquiritigenin. Phytotherapy Research, 2015, no. 29, pp. 969–977.
  7. Tian T., Sun J., Wang J., Liu Y., Liu H. Isoliquiritigenin inhibits cell proliferation and mi-gration through the PI3K/AKT signaling pathway in A549 lung cancer cells. Oncolog. letters., 2018, vol. 16(5), pp. 6133–6139.
  8. Wang K.L., Yu Y.C., Hsia S.M. Perspectives on the Role of Isoliquiritigenin in Cancer. Cancers (Basel), 2021, vol. 13(1), p. 115. DOI: 10.3390/cancers13010115.

About authors

Bortnikova Olga A.
Assistant Lecturer, Department of Pharmacology, Clinical Pharmacology and Biochemistry, Chuvash State University, Russia, Cheboksary (stolga2502@mail.ru; )
Pavlova Svetlana I.
Doctor of Medical Sciences, Head of the Department of Pharmacology, Clinical Pharmacology and Biochemistry, Chuvash State University, Russia, Cheboksary (flavonoid@yandex.ru; ORCID: https://orcid.org/0000-0001-9976-7866)

Article link

Bortnikova O.A., Pavlova S.I. Study of the antitumor activity of isoliquiritigenin on the HeLa, PC3, and MCF-7 Tumor Cell Lines [Electronic resource] // Acta medica Eurasica. – 2021. – №2. P. 28-33. – URL: http://acta-medica-eurasica.ru/en/single/2021/2/4/. DOI: 10.47026/2413-4864-2021-2-28-33.