Single article

Ilyina L.Yu., Sapozhnikov S.Р., Kozlov V.A., Dyachkova I.M., Gordova V.S.

Quantitative Evaluation of Mast Cells Sulfation

Keywords: mast cells, degranulation index, sulfation index, consistency coefficient, liver, kidney, spleen, amyloidosis

The relevance of the study is to develop a new method for evaluating heparin sulfation in mast cells (MCs), based on the calculation of two new relative indicators, in contrast to the currently used method of presenting experimental data in the form of results of MCs direct calculation with various metachromasy variants (Gordon D. S., 1981), which is too cumbersome and difficult to perceive. In addition, the well-known method does not allow to get comparable data obtained on different types of living objects (both animals and individual organs) due to significant quantitative differences in the number of MCs in different species of animals and in different organs of animals of the same species. Based on statistical processing of numerical material contained in a number of previously published articles devoted to the study of MCs tinctorial properties under various experimental effects on two types of animals (laboratory white mice and rats), a method for an objective ranking method for evaluating the degree of MCs sulfation has been developed. It consists in the fact that in histological preparations pre-stained with a metachromatic dye that binds to heparin, the number of MCs with different degrees of sulfation is calculated by the degree of sulfation detected by the degree of metachromasia. The calculated number of MCs is used to calculate the sulfation index (SI) according to the formula developed by the authors. The proposed method of evaluating the activity of MCs makes it possible to compare the degree of heparin maturity and the discharge of biologically active substances into the environment both in each individual cell and in MCs population in a structure, an organ, tissue, regardless of the organ under study or a group of organs and animal species.

References

  1. Artishevskii A.A., Leontyuk A.S., Sluka B.A. Gistologiya s tekhnikoi gistologicheskikh isledovanii [Histology with histological research techniques ]. Minsk, Vysshaya Shkola Publ., 1999, 236 p.
  2. Gordon D.S. Tinktorial’nye paralleli tuchnykh kletok. Makro-mikrostruktura tkanei v norme, patologii i eksperimente [Tinctorial Parallels of mast cells. Macro-microstructure of tissues in norm, pathology and experiment]. Cheboksary, Chuvash State Univesity Publ., 1981, pp. 97–101.
  3. Gordova V.S., D’yachkova I.M., Sergeeva V.E., Efeikina N.B., Moskovskaya O.I. Reaktsiya tuchnykh kletok na postuplenie khimicheskikh elementov s pit’evoi vodoi [The reaction of mast cells to the receipt of chemical elements with drinking water]. Sovremennye problemy nauki i obrazovaniya, 2015, no. 5. URL: www.scince-tbucation.ru/ 128-21918
  4. D’yachkova I.M., Gordova V.S., Sergeeva V.E., Sapozhnikov S.P. Nekotorye adaptatsionnye reaktsii timusa na postuplenie kal’tsiya i kremniya s pit’evoi vodoi [Some adaptive reactions of the thymus to the intake of calcium and silicon with drinking water]. Cheboksary, Chuvash State Univesity Publ., 2014, 140 p.
  5. D’yachkova I.M., Sergeeva V.E., Sapozhnikov S.P. Issledovanie populyatsii tuchnykh kletok timusa pri dlitel’nom vozdeistvii kremniya i kal’tsiya [Study of the population of thymus mast cells under prolonged exposure to silicon and calcium] Vestnik Chuvashskogo gosudarstvennogo pedagogicheskogo universiteta im. I.Ya. Yakovleva, 2010, no. 4(68), pp. 50–55.
  6. Il’ina L.Yu., Efeikina N.B. Sostoyanie populyatsii tuchnykh kletok pochek belykh myshei pri eksperimental’nom amiloidoze [State of the population of white mouse kidney mast cells in experimental amyloidosis]. Acta Medic Eurasica, 2018, no. 2, pp. 50–60. Available at: http://acta-medica-eurasica.ru/wp-content/uploa ds/2018/06/AME_2018_2_str.-50_60.pdf.
  7. Il’ina L.Yu., Kozlov V.A., Sapozhnikov S.P. Reaktsiya tuchnykh kletok pecheni myshei na eksperimental’nyi amiloidoz [Reaction of fat cells to the liver of mice experimental amyloidosis]. Acta Medic Eurasica, 2019, no. 1, pp. 33–43. Available at: http://acta-medica-eurasica.ru/wp-content/uploads/2015/07/AME_2019_1_s.33-43.pdf.
  8. Il’ina L.Yu., Kozlov V.A., Sapozhnikov S.P. Tuchnye kletki pecheni myshei pri eksperimental’nom amiloidoze [Mast cells in the liver of mice with experimental amyloidosis]. Byulleten’ eksperimental’noi biologii i meditsiny, 2019, vol. 168, no. 7, pp. 17-20. DOI: 10.1007/s10517-019-04635-5.
  9. Kozlov V.A., Sapozhnikov S.P., Karyshev P.B. Model’ sistemnogo amiloidoza u molodykh myshei [Model of systemic amyloidosis in young mice]. Byulleten’ eksperimental’noi biologii i meditsiny, 2016, vol. 162, no. 10, pp. 523–527. DOI: 10.1007/s10517-017-3652-y.
  10. Kozlov V.A., Busova O.S. Migratsiya tuchnykh kletok v pochke [Migration of mast cells in the kidney]. Vestnik Chuvashskogo gosudarstvennogo pedagogicheskogo universiteta im. I.Ya. Yakovleva, 2010, no. 1(65), pp. 40–45.
  11. Kozlov V.A., Busova O.S. Tuchnokletochnaya populyatsiya pochki i pochechnoi kapsuly [Mast cell population of the kidney and renal capsule]. Moscow, Shcherbinskaya tipografiya, 2009, 104 p.
  12. Kozlov V.A., Glazyrina O.S., Tolmacheva A.Yu. Vodnaya deprivatsiya vliyaet na ekstraneironal’nyi mediatornyi pul pochek belykh krys i pochechnuyu populyatsiyu tuchnykh kletok [Water deprivation affects extraneuronal mediator pool of the kidneys of white rats and of renal population fat cells]. Nefrologiya, 2003, vol. 7, no. 2, pp. 76–81.
  13. Kondashevskaya M.V. Tuchnye kletki i geparin – klyuchevye zven’ya v adaptivnykh i patologicheskikh protsessakh [Mast cells and heparin are key links in adaptive and pathological processes]. Vestnik RAMN, 2010, no. 6, pp. 49–54.
  14. Kostrova O.Yu., Merkulova L.M., Struchko G.Yu., Mikhailova M.N., Moskvichev E.V. Tuchnye kletki timusa na fone razvitiya adenokartsinomy tolstoi kishki [Thymus mast cells against the background of colon adenocarcinoma development]. Sovremennye problemy nauki i obrazovaniya, 2013, no. 2, p. 43.
  15. Lindner D.P., Poberin I.A., Rozkin M.Ya., Efimov V.S. Morfometricheskii analiz populyatsii tuchnykh kletok [Morphometric analysis of the mast cell population]. Arkhiv patologii, 1980, no. 6, pp. 60–64.
  16. Lyubovtseva L.A., Golubtsova N.N., Gur’yanova E.A., Moskovskii A.V., Agatskin S.A., Lyubovtseva E.V. Kolichestvennyi analiz granulyarnykh lyuminestsiruyushchikh i tuchnykh kletok v organakh immunnoi i neimmunnoi sistem [Quantitative analysis of granular luminescent and mast cells in the immune and non-immune systems] International Journal on Immunorehabilitation. 2000, vol. 2, no. 2, p. 53.
  17. Naumova E.M., Sergeeva V.E. Gistokhimicheskii analiz populyatsii tuchnykh kletok timusa myshei pri vvedenii AKTG1-24 [Histochemical analysis of the population of mouse thymus mast cells with the introduction of ACTH1-2]. Byulleten’ eksperimental’noi biologii i meditsiny, 2004, vol. 137, no. 7, pp. 107–110.
  18. Struchko G.Yu. Uchastie tuchnykh kletok v rannei faze immunnogo otveta timusa na vvedenie rastvorimogo antigena [Participation of mast cells in the early phase of the thymus immune response to the introduction of a soluble antigen]. Immunologiya, 1997, vol. 18, no. 6, pp. 55–56.
  19. Yurina N.A., Radostina A.I. Morfofunktsional’naya geterogennost’ i vzaimodeistvie kletok soedinitel’noi tkani [Morphological and functional heterogeneity and interaction of connective tissue cells]. Moscow, 1990, 398 p.
  20. Yalaletdinova L.R., Gordova V.S., Yastrebova S.A., Sergeeva V.E. Neiroimmunomoduliruyushchie svoistva khorionicheskogo gonadotropina [Neuroimmunomodulatory properties of human chorionic gonadotropin]. Cheboksary, Chuvash State Univesity Publ., 2016, 148 p.

About authors

Ilyina Liliya Yu.
Senior Lecturer, Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (lileaceae@rambler.ru)
Sapozhnikov Sergey P.
Doctor of Medical Sciences, Professor, Head of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (adaptogon@mail.ru)
Kozlov Vadim A.
Doctor of Biological Sciences, Professor of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (pooh12@yandex.ru)
Dyachkova Iraida M.
Candidate of Biological Sciences, Associate Professor of the Department of Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (iraida-djachkova@rambler.ru)
Gordova Valentina S.
candidate of medical sciences, fundamental medicine department associate professor, Immanuel Kant Baltic Federal University, Russia, Kaliningrad (crataegi@rambler.ru)

Article link

Ilyina L.Yu., Sapozhnikov S.Р., Kozlov V.A., Dyachkova I.M., Gordova V.S. Quantitative Evaluation of Mast Cells Sulfation [Electronic resource] // Acta medica Eurasica. – 2020. – №2. P. 43-53. – URL: http://acta-medica-eurasica.ru/en/single/2020/2/6/.