Single article

Ilyina L., Kozlov V., Sapozhnikov S.

Reaction of mast cells in murine liver to experimental amyloidosis

Keywords: mice, red grape wine, liver, prevention, mast cells, fructose, experimental amyloidosis

The article examines the reaction of mast cells (MCs) in murine liver to 1) forming an experimental model of amyloidosis by intraperitoneal introducing an aqueous solution of soy cream substitute TS 9199-004-58706213-10 diluted in proportion of 10 g per 100 ml, 15 times, every second day, in the dose of 0.1 ml per 10 g of murine body mass – 1st group; 2) amyloidogenesis correction by spontaneous consumption of dry red wine supplemented by 5% fructose per os – 2nd group. In mice of the 1st group the percent area (Spercent) of amyloid lesions in Congo-stained histological sections made 17,9±0,1%. The capsule and the parenchyma of mast cells contain α-ortho and β-metachromatic non-degranulating forms in an equal ratio. The proportion of b1 metachromatic MC in the capsule of animals’ liver was 1,8 times higher and in the parenchyma it was 1,5 times higher than in intact animals. In mice of the 2nd group Spercent of amyloid deposition in the liver was less than that in mice of the 1st group and made 2,7±0,13%. In the liver capsule of 2nd group mice, half of all MCs had unsulfated heparin and, unlike the same indicator in the intact group, β2-metachromatic and γ-metachromatic MCs appeared. In the liver parenchyma of 2nd group mice, MCs with immature heparin predominated, β3-metachromatic MCs appeared for the first time in a small amount, the proportion of γ-metachromatic MCs increased. Both in the capsule and in the parenchyma of the liver in group 2 mice, degranulated MCs appeared and the proportion of degranulating forms increased. Thus, the response of MCs to amyloidogenesis is expressed in changing the "maturity" degree of mucopolysaccharides contained in them, and in the amount of forms, including degranulating MCs; red grape dry wine supplemented with 5% of fructose can be a factor in amyloid disease prevention.


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About authors

Kozlov Vadim A.
Doctor of Biological Sciences, Professor of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (
Sapozhnikov Sergey P.
Doctor of Medical Sciences, Professor, Head of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (
Ilyina Liliya Yu.
Senior Lecturer, Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (

Article link

Ilyina L., Kozlov V., Sapozhnikov S. Reaction of mast cells in murine liver to experimental amyloidosis [Electronic resource] // Acta medica Eurasica. – 2019. – №1. P. 33-43. – URL: