Single article

Kozlov V., Sapozhnikov S., Fufaeva A., Aleksandrova V., Petrova Yu., Mizheev M.

Bacteria as a primary source of palatine amyloid

Keywords: amyloid, thioflavin, fluorescence, palatine tonsil, chronic tonsillitis, paratonsillar abscess

The aim of the study was a pathohistological study of palatine tonsil scraping in staining with thioflavin to detect the presence of amyloid in tonsil’s surface structures as a method of minimally invasive diagnosis of amyloidosis. The material was scrapings from palatine tonsils of two young men, taken immediately before a planned (28 y.o.) and urgent (23 y.o., tonsillar abscess) tonsillectomy. Scrapings from the right and left tonsils of both patients were applied to slide glasses, stained with a 0.1% solution of thioflavin S by top irrigation and covered with coverslips. In an hour after taking the scrapings, they were examined under a luminescent microscope Lumam, fluorescence intensity of luminous objects was measured with the help of the FMEL 1A luminometer in millivolts. In both cases in tonsillar scrapings fluorescent with dark green or yellow-brown caseous masses (fluorescence intensity of 84±17 mV) were detected as well as: 1) single fluorescent epithelial cells (10±3); 2) non-fluorescent irregularly shaped large cells with a slightly fluorescent nucleus in the form of filamentous structures that are distinguished by a black cytoplasm against a fluorescent background; 3) dental amoebae with a cytoplasm and from one to four nuclei, fluorescing in green (44±49 and 86±94, respectively); 4) colonies of rod-like bacteria with bright golden-brown fluorescence (105±24); 5) cocci colonies (tetracocci and diplococci) fluorescing with green and golden yellow color (94±29 mV), and 6) single coccal forms. Based on the results of the study, conclusions are drawn: 1) mixed infection accompanying chronic tonsillitis, produces amyloid, which is contained, among others, in caseation of tonsillar crypts; 2) bacterial amyloid may be an incentive for forming amyloid masses inside a tonsil; 3) examination of scrapings and oral biopsy specimens for the purpose of identifying systemic amyloidosis is most likely to be an inadequate method of investigation; 4) detection of amyloid in a tonsillar scraping using thioflavin S can be a differentiating method of early diagnosis, testifying to the need for urgent tonsillectomy to prevent age-related systemic amyloidosis.

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About authors

Kozlov Vadim A.
Doctor of Biological Sciences, Professor of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (pooh12@yandex.ru)
Mizheev Mikhail Borisovich
highest category doctor, otorhinolaryngological unit head, Emergency Hospital, Russia, Cheboksary (mmbmbi@yandex.ru)
Sapozhnikov Sergey P.
Doctor of Medical Sciences, Professor, Head of Department Medical Biology with a Course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (adaptogon@mail.ru)
Fufaeva Alena I.
Post-Graduate Student of Biology and Microbiology Department, Chuvash State University, Russia, Cheboksary (priffetik@bk.ru)
Petrova Julia Victorovna
3th year student, Chuvash State University, Russia, Cheboksary (yulya-cbx@mail.ru)
Alexandrova Vera Yurievna
3th year student, Chuvash State University, Russia, Cheboksary (verochka789@mail.ru)

Article link

Kozlov V., Sapozhnikov S., Fufaeva A., Aleksandrova V., Petrova Yu., Mizheev M. Bacteria as a primary source of palatine amyloid [Electronic resource] // Acta medica Eurasica. – 2018. – №3. P. 24-33. – URL: http://acta-medica-eurasica.ru/en/single/2018/3/4/