Ilyna L., Efeykina N.
The state of mast cells population in mice kidneys in experimental amyloidose
The aim of the study was to study the reaction of mast cells (MS) population in the kidneys to formation of an experimental model of amyloidosis and correction of amyloidogenesis by means of oral spontaneous consumption of dry red wine. In the experiment 19 white outbred mice aged 30 days were used, they were divided into four groups – intact group of 5 mice (I),mice of the second group (CM), third (VM) and fourth (VF) groups (3 mice in each group) had simultaneously generalized amyloidosis simulated by intraperitoneal injection of an aqueous solution of 10 g per 100 ml of soy cream substitute TU 9199-004-58706213-10 15 times every other day at a rate of 0.1 ml per 10 g of mouse weight. The second group (control model – CM) served as control for forming generalized amyloidosis model. VM group received dry red vein Cabernet (Crimea) per os in the mode of free access, VF group received wine as well with supplement of 5% fructose. The control of the model formation and the severity of amyloidosis being formed were controlled by staining with congo red, MS were detected by Unna staining, and then the degree of heparin sulphatedness in mast cells and their tendency to degranulate were assessed. Histological examination of the frontal renal sections revealed a marked amyloid damage of kidneys of CM group mice, in VM and VF groups the morphological structure of sections did not differ from the one in intact mice, small deposits of amyloid were observed in individual arterioles as well as in the form of dust in some glomeruli and cortical tubules. Amyloidosis modeling increased the sum of β3 and γ-metachrome MS forms in the kidney capsule and in cortical areas from 3,68% in intact animals to 20,35% in the CM group (p = 0,019, z test).In CM group, there was an increase in the number of total destroyed MS from 2,2% in intact mice to 9,5% in CM group (p = 0,0000, z- test). Wine consumption supported the cytological status of MS population in these mice at a level comparable to intact mice: sulphated forms in VM group – 3,45%, in VF group – 9,38% (p = 0,0000, z- test, intact and VM). Disappearance of totally destroyed MS in VM and VF groups was observed. The proportion of T0 cell forms increased 1.56 times (p = 0,0597, z-test) in VM group and 2,1-fold in VF group (p = 0,0013, z -test). It was concluded that mast cells were sensitive markers reacting to amyloidogenesis both by changing the degree of "maturity" of mucopolysaccharides contained in them, and by changing the number of forms, including degranulating cells; red grape dry wine can be a factor for preventing amyloid disease.
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- Efeikina Nadezhda B.
- Candidate of Biological Sciences, Associate Professor, Department of Medical Biology with course of Microbiology and Virology, Chuvash State University, Russia, Cheboksary (firstname.lastname@example.org; )
- Ilyina Liliya Yu.
- Senior Lecturer, Department of Medical Biology with a course in Microbiology and Virology, Chuvash State University, Russia, Cheboksary (email@example.com; )
Ilyna L., Efeykina N. The state of mast cells population in mice kidneys in experimental amyloidose [Electronic resource] // Acta medica Eurasica. – 2018. – №2. P. 50-60. – URL: http://acta-medica-eurasica.ru/en/single/2018/2/8/.