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Danilova I.M., Pavlova S.I., Khobrakova V.B.
Immunomodulatory Properties of Glycyrrhizinic Acid in a Model of Cyclophosphamide-Induced Toxicity and Immunosuppression
Keywords: glycyrrhizinic acid, immunosuppression, toxicity, cyclophosphamide, pharmacology, oncology, cytostatics, antitumor drugs
Triterpenoid glycyrrhizic acid, which is part of licorice root extract, has a wide range of biological effects. It was previously revealed that due to glycyrrhizic acid the extract increases the survival rate of animals against the background of cyclophosphamide-induced toxicosis. Since cytostatics cause a wide range of side effects, including pronounced suppression of the immune system, further investigation of glycyrrhizic acid's effect on immunity is of interest. The purpose of the study was to evaluate the effect of glycyrrhizic acid on the immune response in vitro and in experimental animals against the background of cyclophosphamide administration. Materials and methods. The study was carried out in vitro on mice splenocyte culture, as well as on models of cyclophosphamide-induced toxicity and immunosuppression using cyclophosphamide at the dose of 450 mg/kg and 250 mg/kg. The survival rate of animals, the morphological presentation of splenocytes, the content of acid phosphatase, the state of cellular and humoral immunity and phagocytic activity were evaluated. Research results. Glycyrrhizic acid did not inhibit proliferation of mitogen-activated lymphocytes. Administration of glycyrrhizic acid was found to improve the morphological presentation of the spleen by virtue of increasing the size of lymphoid follicles and reducing foci of necrosis against the background of cyclophosphamide-induced toxicosis and to reduce the amount of acid phosphatase. Against the background of cyclophosphamide-induced immunosuppression, administration of glycyrrhizic acid increases the index of delayed hypersensitivity reaction, increases the number of antibody-forming cells compared with the control, improves the phagocytic activity. Conclusions. Glycyrrhizic acid improves the indices of the immune response both in vitro and in modeling cyclophosphamide-induced immunosuppression in laboratory animals. Glycyrrhizic acid improves the morphological presentation of the spleen, as well as enhances cellular, humoral immunity, the phagocytic response against the background of cyclophosphamide administration.
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About authors
- Danilova Irina M.
- Senior Lecturer, Department of Pharmacology, Clinical Pharmacology and Biochemistry, Chuvash State University, Russia, Cheboksary (medicine345@mail.ru; ORCID: https://orcid.org/0009-0000-7836-8081)
- Pavlova Svetlana I.
- Doctor of Medical Sciences, Head of the Department of Pharmacology, Clinical Pharmacology and Biochemistry, Chuvash State University, Russia, Cheboksary (flavonoid@yandex.ru; ORCID: https://orcid.org/0000-0001-9976-7866)
- Khobrakova Valentina B.
- Doctor of Biological Sciences, Associate Professor, Head of the Laboratory of Experimental Pharmacology, Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences, Russia, Ulan-Ude (val0808@mail.ru; ORCID: https://orcid.org/0000-0002-4689-5706)
Article link
Danilova I.M., Pavlova S.I., Khobrakova V.B. Immunomodulatory Properties of Glycyrrhizinic Acid in a Model of Cyclophosphamide-Induced Toxicity and Immunosuppression [Electronic resource] // Acta medica Eurasica. – 2024. – №2. P. 50-63. – URL: https://acta-medica-eurasica.ru/en/single/2024/2/7/. DOI: 10.47026/2413-4864-2024-2-50-63.